what research questions might require the use of nonhuman animals? how would you ensure the proper treatment of these animals?

Many researches require use of animals because those could not be done on human. To protect the animals used in such research, there are very strict guidelines. A quick search online shows published documents from American Psychological Association, National Institute of Health and other agencies advising on the topic. Researchers must follow such guidelines when they use animals for their researches.

its c because i have that test too