Check all the ways listed below that ensure accurate reading of volume from a graduated pipette. tapping the base of the pipette on the table using subtraction to determine the volume dispensed making sure that you read the volume with the liquid inside the pipette at eye level reading only the final volume of liquid after dispensing ensuring that air bubbles are not in the column of liquid inside the pipette

Explanation:

The pipette is a widely used laboratory material, and its main function is to transport precise quantities of liquid material. They are used, for example, in various medical examinations and in the study of molecular biology. There are several types, such as the graduated pipette, the volumetric pipette, the automatic pipette and also the micropipettes (for very small amounts of liquid). Using a pipette requires careful attention not to use wrong volumes of liquid to be used. For this reason, it is correct to say that when handling a pipette we must:

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure