How did the separation of earths continents effect biological evolution?

Last week i was assigned a thematic. we have groups of three but the people i was assigned with haven't been in school for days. i would appreciate if anyone can me with my thematic . i highlighted most of the important steps and i circled my thematic topic. in desperate need : ( its due on february 19

Acquired mutations can result from radiation pollution toxins inheritence select all that apply

In which layer do organisms on earth love?