Aplasmid vector contains a single ecori site in the polylinker (multiple cloning site). a gene of interest is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori, and ligate the two. how many ecori site(s) is/are present in the recombinant dna molecule and how many band(s) would you see when you run your digest of recombinant dna on a gel? (assume that there is no internal ecori site in the insert dna.)

cramming before a final is bad because it makes a student's stress level higher. students who embrace cramming to get through a final exam week find themselves struggling to perform consistently, once the brain adjusts to prolonged sleep deprivation.

burning of wood and coal

explanation:

the panel also concluded there's a better than 95 percent probability that human-produced greenhouse gases such as carbon dioxide, methane and nitrous oxide have caused much of the observed increase in earth's temperatures over the past 50 years.