Four restriction digestion reactions were performed using the plasmid containing the ligated pcr product and one or more restriction enzymes. the result of each restriction digest was then run on an agarose gel. use the plasmid map from part e to you determine which restriction enzymes were used for each reaction.

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

When a human or animal consumes food, the carbon in that food is most likely to be converted into which of the following elements? a. carbon remains carbon b. nitrogen c. oxygen d. hydrogen

Under the soviet system, the government a. controlled all forms of communication. b. allowed newspapers to print whatever they wanted. c. controlled editorials but not the reporting of news. d. encouraged access to a wide variety of news sources. select the best answer from the choices provided a b c d