How do substrate concentration and ph affect enzyme-controlled reactions

For example, pH can have an effect of the state of ionization of acidic or basic amino acids. Acidic amino acids have carboxyl functional groups in their side chains. Basic amino acids have amine functional groups in their side chains. If the state of ionization of amino acids in a protein is altered then the ionic bonds that help to determine the 3-D shape of the protein can be altered. This can lead to altered protein recognition or an enzyme might become inactive.

Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that either the substrate connot bind to the active site or it cannot undergo catalysis.

In geneal enzyme have a pH optimum. However the optimum is not the same for each enzyme.

An ecosystem is in a certain area and includes living and non living plus there is weather conditions and a biome is a large amount of area with similar climate.so ecosystem is based on weather and biome is based on the average climate