Recall the procedure for measuring tyrosinase activity. what is the purpose of the phosphate buffer

Phosphate buffer is a buffer used in many types of reactions with the function to maintain constant pH value.

This is important because enzymes’ activity is affected by changes in pH. There is an optimum pH for every enzyme that can be defined as most favorable pH value or the point where the enzyme is most active.  Phosphate buffer is often use in the measurement of enzyme’s activity because  it is isotonic and non-toxic to most cells.

1. amino acid refers to the fundamental building block of proteins, comprising of an amino group and a carboxyl group.

2. catalyst refers to any substance, which increases the rate of chemical reaction without being used up in the reaction.

3. a complex molecule containing he genetic code is dna or deoxyribonucleic acid.

4. an enzyme refers to a molecule of protein, which is unique in accelerating up a particular chemical reaction in the biological systems.

5. nucleic acid refers to an sequence of sugars, phosphates, and nitrogenous bases, for example, dna and rna.

6. a protein refers to a large, composite organic molecule comprising of a huge number of amino acids associated together with the of peptide bonds.

7. rna refers to a nucleic acid that takes part in the production of proteins.

8. substrate refers to a compound, which takes part in a chemical reaction, and is chemically modified by the reaction.