If we have a plate that has 2500 colonies on it, can we use this plate to calculate cfu's

No, the solution with bacteria in it needs to be diluted in order to be in the range of 30 – 300.

A colony-forming unit –CFU is used to estimate the number of viable (ability to divide by binary fission) microbes in a sample.  

A sample of microbes is diluted and spread on a plate. Then, the number of colonies is counted, assuming that each colony have grown from a single colony-forming unit, or CFU.

Plates with more than 300 colonies are difficult to count while those with less than 30 colonies give statistically unreliable numbers of colonies to count.

it has to be understood that the sea water contains a huge amount of sodium and chlorine ions and if they enter the cell of the atlantic cod fish, the cell will shrivel and the fish will die. to avoid such a condition, the fish uses active transport for the purpose of removing the ions against the gradient.

explanation:

the atlantic cod is not a maritime osmoconformer. its native application of salts is less than that of the seawater it lives in. it is continually falling water from the gills to the ocean via osmosis.

countering that, the fish is going to be swallowing seawater. but since its internal collection of salts is lower than seawater, the fish will want to go free of excess salt, and since that's going upon a gradient, then active transport, requiring energy, must be used to pump out the salt. much marine fish use the organs to filter out the salt and these fish discharge very straight urine.

rising temperatures because when water vapor in the air is cooled, it condenses. it turns back into a liquid and attaches to dust particles in the air. these very small water droplets group together to form clouds