Organisms share many conserved core processes and features, including transcription and translation using a uniform genetic code. scientists have used these shared processes and features in biotechnology. for example, for the process of some transformations, a plasmid is constructed when a eukaryotic gene of interest is added with an antibiotic resistant gene such beta-lactamase, which is used for ampicillin resistance. this plasmid is then inserted into a prokaryotic bacterial cell, such as e-coli, through a transformation process that leads to the production of the product protein from the eukaryotic organism. to culture the bacteria and obtain the protein product, the bacteria must grow. select the appropriate condition to determine if the plasmid has entered the e-coli bacterial cell.