Aeukaryotic gene has "sticky ends" produced by the restriction endonuclease ecori. the gene is added to a mixture containing ecori and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. the plasmid has one recognition site for ecori located in the tetracycline resistance gene. this mixture is incubated for several hours, exposed to dna ligase, and then added to bacteria growing in nutrient broth. the bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. bacteria that do not take up any plasmids would grow on which media?

Two critical interventions to turn around the opioid crises are:

Is a measure of the resistance to flow. a high liquid has a high resistance to flow and flows slowly. the ancients thought everything in the world was made of 4 we now know that there are 94 naturally occurring and scientists have created another 24 i am certain they will create even more. honey flows slowly because it has a high to flowing. a can be separated by physical means because it contains more than one pure substance and 2 pure substances are not chemically bonded to each other. a cannot be separated by physical means. all matter is made up of all elements are with the same number of protons. if it is just a single or many bonded together, if all of them have the same number of protons, it is an element. in a piece of pure iron metal, all the are joined together, that piece of iron metal is called elemental iron. a single of iron is called elemental iron. a mixture has differences from place to place. we might need a microscope to see them or they might be obvious to the unaided eye. there are surfaces separating it into different phases. a mixture is the same everywhere. it is uniform. there are no surfaces separating it into different phases. if different kinds of atoms (different elements) are bonded together by their electrons, it is called a there are physical means of to isolate the different pure substances in a mixture and there are chemical means of to isolate the different elements in a compound. 1. element 2. compound 3. mixture 4. heterogeneous 5. homogeneous 6. pure substance 7. atoms 8. separation 9. viscosity 10. resistance

Which of the following is the best definition of a eukaryota? a. a one-celled organism that does not have a membrane-bound nucleus b. a one-celled organism that does not have a cell wall c. an organism whose cells each contain a membrane-bound nucleus d. an organism whose cells each have a cell wall