Answer will give 75 points

1. eight double-stranded dna molecules resulted after three complete cycles of a single dna double helix. how many molecules will result after 10 cycles? 20 cycles? 30 cycles? (hint: you’ll need a calculator that can do logarithmic functions)

2. how do the amplified dna strands compare with the original dna strands? (answer should include: are the sequences the same/different? length the same/different? )

3. after 30 cycles, what percent of the dna in the test tube would be like the original dna strand? what percent would be like the target segment?
could dna be amplified with only one primer? why or why not?

4. primers will sometimes complement each other and create a dna product. this creates a problem when trying to determine what came from your target dna and what was created by the primer complementation.

5. what could you add when you design your pcr experiment to make sure that only dna product you are seeing is coming from your target dna? (hint: what do you need for any experiment? )