What effect would using a different restriction enzyme have in human gene engineering?

A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA ...

answer; ////increased water pollution; ///////////////////////

the second type of galaxy is called a barred spiral, which has a thing called a bar straddling the nucleus