Denaturation of dna is a necessary step in southern blotting procedure because it separates double stranded dna into single stranded dna. true or false?

True

Explanation:

Denaturation of DNA is a process in which DNA loses its structure (secondary, tertiary, quaternary). It is usually achieved via the use of high temperature, strong acid or base, concentrated salt etc. The denaturation of the double-stranded DNA is used in Southern blotting procedure so that single strands can bind to the membrane and become ready for hybridization.

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