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Eosin-methylene blue agar, or emb, is a commonly used bacteriological medium for growing gram-negative bacteria from complex environments. it contains two dyes, eosin and methylene blue, as well as sugar lactose. eosin is a ph indicator that changes color when the medium is acidic, while methylene blue inhibits the growth of most gram-positive bacteria. if an organism consumes lactose, acid will be produced, causing the bacterial colonies to change color. based on this description, this medium
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