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Biology, 13.09.2019 00:20 eyeneedalife

When 1 million cells of a culture of haploid yeast carrying a met— auxotrophic mutation were plated on petri plates lacking methionine (met), five colonies grew. you would expect cells in which the original met— mutation was reversed (by a base change back to the original sequence) would grow on the media lacking methionine, but some of these apparent reversions could be due to a mutation in a different gene that somehow suppresses the original met— mutations. how would you be able to determine if the mutations in your five colonies were due either to a precise reversion of the original met— mutation or to the generation of a suppressor mutation in a gene on another chromosome?

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