Assume that you have 1 ml of a solution of amylase (an enzyme) at a concentration of 15 mg protein/ml. calculate the volume of diluting buffer that you would have to add to 1.0 ml of the amylase stock solution if you wished the final concentration of the solution to be 345 µg protein/ml.

42,5 mL

Explanation:

We need to use the serial dilution formula beacuse we start with a stock concentrate solution and we need to prepare a new less concentrated one.

DF in the dilution factor, Vi is the initial volume and Vf is the final volume.

The first step is to have the same measurment unit so we need to convert 345 µg to mg.

we know that 1 µg equals 0,001 g, hence:

now the final volume is 0,345 mg  protein/ mL and the inital volume is 15mg protein/mL, both of them are in the same unit so we can use the formula

Now since the question said that we already have 1.0mL of the amylase stock solution we need to subtract that 1.0mL to the 43,5 mg protein/mL

So, we need 42,5 mL of diluting buffer if we want a final concentration of 345 µg protein/mL (0.345 mg protein/mL)

eukarya because is is like alche and b is bacteria and d is animals

explanation:

correct answer: understand the different control methods available

integrated pest management is an strategy that is based on the ecosystem and try to prevent the pest and the damage caused by them by applying combination of technique like habitat manipulation, biological control and use of resistant varieties.

the main aim of the integrated pest management is not to completely eliminate the pest but to strengthen the ecosystem so that conditions are favorable for plants and unfavorable for pests.