Aproblem with of cell number is that dead cells also scatter light. select one:
a. viable counts
b. direct microscopic counts incorrect
c. spread plate counts
d. optical density measurements
e. pour plate counts

The correct answer is d. optical density measurements.

Explanation:

The optical density of a culture is measured to know the concentration of the bacteria in the culture. Spectrophotometer is the device used to measure the optical density of the cell.

The more the optical density more will be the concentration of bacteria and more will be the number of bacteria. Optical density is based on absorbance and scattering of light. So even dead cells can absorb and scatter the light.

So high optical density does not mean that there are more viable cells in the culture, therefore, it is a problem with optical density measurements of cell number is that dead cells also scatter light.

light dependent reactions:

light independent reactions:

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