In the early days of research on protein synthesis, some scientists observed that their most highly purified ribosome preparations, containing almost exclusively single ribosomes, were less active than preparations that were less highly purified. what is the likely explanation for this observation?

a. single ribosomes are less active than polysomes, which have been removed during the preparation of the highly purified sample.
b. the high level of purificaton causes the shape of the mrna binding site to change so that the ribosome cannot move along the mrna as efficiently.
c. the high level of purification likely caused denaturation of the ribosomes.
d. purification damages the trna binding site, reducing the ribosome activity.