You have now added both pcr primers and successfully run the pcr, but you notice that the quantity of product generated is low. the graduate student you work with mentions that your expected product is quite long and that you may want to alter your pcr conditions to increase efficiency of product generation. which of the following would increase the amount of long pcr product generated?
a. increasing the annealing temperature when primers hybridize to the template dna
b. increasing the elongation temperature when dna polymerase synthesizes the new dna
c. increasing the elongation time when dna polymerase synthesizes the new dna
d. increasing the annealing time when primers hybridize to the template dna

the answer is otpion c) understanding

he wants to be heard one more time. he wants to be understood. he needs the truth one more time.

hypoxemia refers to a below usual concentration of oxygen in the blood, mainly in the arteries. hypoxemia is an indication of an issue associated with circulation or breathing and may lead to various symptoms like shortness of breath.  

the condition is identified by determining the level of oxygen in the blood sample obtained from an artery. it can also be predicted by determining the oxygen saturation of the blood with the application of a pulse oximeter.  

the normal arterial oxygen is about 75 to 100 mm hg. the values underneath 60 mm hg is generally considered with the requirement for supplemental oxygen. the normal pulse oximeter readings generally range between 95 to 100 percent. the values under 90 percent are regarded as low.  

in the given case, the oxygen saturation is measured at 93 percent, hence, there is no need to administer oxygen, that is, one should withhold oxygen.