Forward primer: 5'-agtctactcgtaaccggttacc-3' reverse primer: 5'-taaggcatcatggtaaccggtt-3' the two primers above have the melting temperatures between 55c-80c. what is the reason that these two primers will not bind effectively to the region of dna that we want to amplify?

Short answer is primers are partially complementary.

Explanation:

Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'

Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'

When we write reverse primer 5' to 3' we can easily see that

3'-TTGGCCAATGG---5' is complementary to the forward primers'

5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.

preservation because he preserves part of the land and preserves means save

explanation: