Each of the following below are true of enzymes except select one: a. they can be used over and over. b. they may or may not require cofactors. c. their active site is specific to the substrate. d. they increase the activation energy of a reaction. e. their action may involve minerals.

The answer option which isn't true of enzymes is: d. they increase the activation energy of a reaction.

An enzyme can be defined as a biological catalyst that typically speeds up (increases or accelerates) the rate of a chemical reaction, by lowering (decreasing) the activation energy of its constituent chemical elements (reactants).  Thus, the rate of any chemical reaction is faster when its activation energy is decreased, reduced or lowered.

Basically, some of the characteristics of an enzyme are:

In conclusion, an enzyme catalyzes (speeds up) the rate of a chemical reaction by lowering (decreasing) its activation energy rather than increase it.

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option D.

Explanation:

Enzyme are protein that act as catalyst in living organisms thereby regulating the rate of chemical reaction without itself being changed in the process.

Enzymes lowers the activation energy of a reaction because it not possible for it to affect the overall enthalpy of a reaction and in doing this they increase the rate of a reaction, helping it to occur faster.