In 3-5 sentences, relate the impact of the industrial use of water for hydraulic fracturing (fracking) with the availability and quality of water.

Fracking has adverse effect on water resources. The most potent danger lies in the fact that the fracking fluid may flow back to the surface and contaminate water.The  fracking fluid contains toxins and carcinogen along with sand. This fluid when mixes with fresh water resources by any chance will prove hazardous. And hence degrade the availability and quality of potable water.

Explanation:

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.