You have made a specific dna probe that will bind to a key sequence on the dna of neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. however, upon testing it out against a known neisseria gonorrhoeae culture, you find that it does not work. which of the following is a possible explanation for this negative result? a. you forgot to label the probe sequence with a reporter molecule.

b. you forget to digest the probe with restriction endonucleases

c. you forget to add the mrna to the dna probe sample.

d. you forgot to incubate the test at the 75c temperature required for hybridization.

The correct answer is i forgot to label the probe with a reporter molecule.

Explanation:

Reporter molecule is a specific type of molecule that reports the success of cloning.

      In this experiment to test the presence of N gonorrhoeae by using the DNA probe that was developed by me should be at first labelled probe sequence with a reporter gene such as lucciferase protein.

       When this reporter molecule labelled DNA probe will bind to the target DNA sequence of N gonorrhoeae at that time by using the substrate specific for that reporter molecule will help me to notice the process of hybridization.

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parasites can detrimentally affect their host, but also must not allow their host to die before they can complete their life cycle. some parasites have complex life cycles with many different hosts, and have had to find ways of persisting in many different environments during the change from one host to another. ectoparasites live outside the body of the host, and can more easily  disperse, but are  more exposed to predators, parasites, parasitoids. endoparasites live inside the body of the host, and are therefore protected from the outside environment. but they can be  attacked by the host's immune system. they have therefore developed  mechanisms for dispersal such as  complex life cycles.