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Biology, 21.11.2019 01:31 natalie2sheffield

P-elements are a type of transposon commonly used in drosophila (fruit fly) research. a common marker for p-elements is the miniwhite + gene, an engineered version of the white locus. flies mutant for the natural white have white eyes instead of wild-type red eyes. a miniwhite + gene on a p-element in a white mutant will produce eye pigment, and will act as a dominant marker indicating that the p-element is present. p-elements used for research are nonautonomous, meaning that they do not encode their own transposase. the transposase is supplied using a second locus, where the transposase enzyme is no longer within a transposon and thus is immobilized (meaning that it cannot move itself). for flies with both a nonautonomous p-element and immobilized transposase, the p-element can move within the genome. a researcher is interested in screening for p-elements inserted into a region on chromosome 3. to produce new insertions, she crosses females homozygous for a miniwhite + p-element on the x chromosome with males homozygous for immobilized transposase on chromosome 2.the researcher then crosses the f1 males (xp{miniwhite+}/ y; transposase / +) to female flies that are homozygous for the white mutation. she selects f2 males that have eye pigment present (indicating that they have a p {miniwhite +} insertion somewhere in their genome).why does the researcher select these f2 males? select all statements that are true. a) in this cross, most f2 males will have eye pigment because they inherit the xp{miniwhite+} chromosome with a p-element that did not move. b) in this cross, most f2 males will have no eye pigment because they cannot inherit the x chromosome that had the original p {miniwhite +} insertion on it. c) in this cross, f2 males with eye pigment result from the p-element moving to a new location on an autosome. d) in this cross, f2 males with eye pigment result from the p-element remaining at its original location on the x chromosome. to find insertions into a specific region on chromosome 3, the researcher separately crosses individual f2 male flies with eye pigment to females heterozygous for a deletion on chromosome 3. since this deletion is homozygous lethal (as most large deletions are), she uses flies that have one copy of a normal chromosome 3 as well as one copy with the deletion. the normal (non-deleted) chromosome 3 also carries a dominant mutation called serrate, which produces notched wings. (this non-deleted chromosome also does not allow for recombination with the chromosome with the deletion).the females are also homozygous for the white mutation, meaning that any progeny with eye pigment will have a p-element insertion with its miniwhite + marker. in this way, the researcher hopes to find p-element insertions in her screen that do not survive when paired with the deletion -- indicating that the insertions are in the region spanned by the deletion, and have caused a mutation in a gene essential for survival. when the researcher examines the progeny with eye pigment that result from these crosses, she finds two different categories of flies. some crosses produce flies with eye pigment and notched wings, as well as flies with eye pigment and normal wings. other crosses produce flies with eye pigment and notched wings, but no flies with eye pigment and normal wings. what might explain these results? select all statements that are true. a) progeny with eye pigment have a p {miniwhite +} insertion they inherited from their father. b) progeny with notched wings inherited the non-deleted chromosome 3 from their mother. c) progeny with normal wings inherited the chromosome 3 with the deletion from their mother. d) progeny with notched wings and eye pigment have both a p {miniwhite +} insertion and the chromosome 3 with the deletion. e) progeny with normal wings and eye pigment have both a p {miniwhite +} insertion and the chromosome 3 with the deletion. f) a cross that produces progeny with normal wings and eye pigment indicates that the p-element insertion has disrupted an essential gene in the region spanned by the deletion. g) a cross that does not produce progeny with normal wings and eye pigment indicates that the p-element insertion has disrupted an essential gene in the region spanned by the deletion.

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