Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (gfp) and the insertion of the plasmid into a bacterial cell by placing the steps in order
-ligate together the gfp gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation
-use pcr to amplify the gene for gfp
-perform a restriction digestion of the gfp gene and the plasmid
-plate the bacterial cells, and screen for positive transformants