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Biology, 28.11.2019 04:31 maustin5323

Which of the following statements about pcr is/are false? there may be more than one correct answer.

a) pcr reactions contain dna template, primers, deoxyribonucleotide triphosphates, and heat- resistant dna polymerase
b) each pcr cycle includes three steps: denaturation, annealing, and extension
c) after each pcr cycle, the amount of dna increases in a linear manner
d) one limitation of pcr is that it requires prior knowledge of the sequence to be amplified
e) pcr is a very sensitive technique and can amplify target sequences present in extremely low copy number
f) the single-stranded dna primers must anneal to opposite strands of template dna at distant sites, with their 5′ ends directed toward each other

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Which of the following statements about pcr is/are false? there may be more than one correct answer...
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