Biology, 30.11.2019 02:31 nanagardiner08
Suppose you have identified a mutant strain of yeast that cannot grow without leucine added to the growth medium. you know that the leu- phenotype results from a single mutation, but you do not know where in the yeast genome the mutation occurred. which of the following strategies would you use to clone the wild-type gene corresponding to the mutant allele? only one answer correct will be considered correct.(a) pcr amplification of the gene using flanking primers(b) screening of a yeast-cdna expression library using an antibody probe(c) complementation of the leu- phenotype with a yeast genomic plasmid library(d) hybridization of a dna probe to a southern blot(e) hybridization of a dna probe to a northern blot(f) screening of a yeast-genomic λ phage library using a dna probe
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Suppose you have identified a mutant strain of yeast that cannot grow without leucine added to the g...
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