Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor viii. not all steps will be placed. isolate the human gene that produces factor viii-generate cdna of the factor viii gene using reverse transcriptase- transform the vector into e. coli bacterium-the factor viii protein is isoloated from human tissues-insert the factor viii cdna into a bacterial vector near a promoter site-human dna is introduced into the bacterial cell using direct injection by a small needle-isolate the mrna of the factor viii genee. coli expresses factor viii

Isolate the mRNA of the factor VIII.

Generate cDNA of the factor VIII gene using reverse transcriptase.

Insert the factor VIII cDNA into a bacterial vector near a promoter site.

Transform the vector into E. coli bacterium.

E.coli expresses factor VIII.

Explanation:

This is the way to produce a human protein in a bacterial model, since bacteria does not perform the splicing process is better to generate a cDNA copy of the mRNA, since it has the introns already removed. If you use the gene from the genomic DNA, it will have the exons and the introns, and the bacteria is not able to process the RNA to a mature state.