Relative to the transcription start site, where would the promoter and terminator be located?

Promoter is located upstream and terminator is located downstream relative to the transcription start site.

Explanation:

Transcription start site is the site from where the transcription starts which is denoted by +1. The sequence of nucleotides which is present backword to them called upstream sequence and nucleotide sequence which is present in the forward direction of transcription start site are called downstream sequence.

Promoter sequences are present upstream to the transcription start site and are denoted with a minus sign and terminator sequence is present downstream to the transcription start site.

answer; both surfaces of the membrane are nonpermeable