8) what is the manipulated variable in this experiment?

As the variables are not given, let us help with general explanation on manipulated variable.

Explanation:

A manipulated variable is an independent variable. In a scientific experiment, an independent variable can be described as a variable which can change naturally or which is changed by the researcher to check its effect on the dependent variable. The dependent variable is the variable which is under study and being tested.

For example, to check the difference in photosynthesis rate by light, the amount of light will be the independent variable and the rate of photosynthesis will be the dependent variable.

gold i'm pretty sure i might be wrong

ans.

dna fingerprinting is defined as a process that determines the dna characteristics of an individual. these dna characteristics are unique among individuals as their fingerprints, so it is known as dna fingerprinting.

steps of dna fingerprinting includes extraction of dna from sample, amplification of dna, restriction digestion of amplified dna, and then analysis of dna by gel electrophoresis, respectively.

thus, the next step performed by the scientist is 'to cut the amplified dna (obtained by polymerase chain reaction) by restriction enzymes that cleave the dna into fragments and their analysis.'