The protein pictured is bovine insulin. Determine the residue number and the size of the fragments that would be generated upon treatment with following proteases (1) trypsin, (2), chymotrypsin and (3) BrCN; (a) without DTT and (b) with DTT. (Where DTT breaks disulfide bonds).

The table presents the average day and night temperatures in five cities. it also reveals whether a city receives substantial rainfall (wet climate) or little rainfall (dry climate). which city’s rocks are likeliest to experience frost wedging, and why? a) city a because the consistently subzero temperature would prevent water from melting b) city b because it is a wet region and the temperature fluctuates around the freezing point c) city c because it receives plenty of rain fall and the weather is moderately cool d) city d because the hot and dry weather would cause rocks to absorb water