You put a culture of cells on the shaker at 37°C. You had intended to come into lab the next day to harvest the cells and purify plasmid DNA from them. Instead, you wake up the next day with the flu. You are home for 2 days with a fever and chills. When you drag yourself back into lab, 3 days after you put the culture on the shaker, you check your cells. You have a dense growth and an OD600 greater than 1.0. Should you attempt to purify the plasmid DNA from this culture?

In this situation, we should not attempt to purify DNA from this culture. When you incubate culture on a shaker for more than a day, cells keep growing and result in dense growth. The colonies become clustered due to longer incubation on shaking making it difficult to purify plasmid DNA from densely-grown cells.

this is a good question! i'll do some research and come back when i have the answer!

he movement of air through earth's or any planet's atmosphere is called wind, and the main cause of earth's winds is uneven heating by the sun. this uneven heating causes changes of atmospheric pressure, and winds blow from regions with high pressure to those with low pressure.

i hope this : )