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The oxidation of malate by NAD+ to form oxaloacetate is a highly endergonic reaction under standard conditions (DG’o = +7.0 kcal/mol). The reaction proceeds readily under physiological conditions:a) Why?b) Assuming an [NAD+]/[NADH] ratio of 8 and a pH of 7, what is the lowest [malate]/[oxaloacetate] ratio at which oxaloacetate can be formed from malate?
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