You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction, but instead are light purple. Your staining procedure was performed correctly. What is your best explanation as to why the bacteria have stained this way?

A. The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.
B. There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
C. The specimen did not undergo heat fixation before staining; therefore, the primary dye does not stick properly to the wall.
D. These are mutant bacteria which cannot be stained like other bacteria.

Correct answer is option "B"

Explanation:

Gram staining depends on the capacity of microscopic organisms cell divider to holding the precious stone violet color during dissolvable treatment. It is quite often the primary test performed for the distinguishing proof of microscopic organisms. The essential stain of the Gram's technique is precious stone violet. Precious stone violet is now and then subbed with methylene blue, which is similarly compelling. The microorganisms that hold the crystal violet-iodine complex seem purple dark-colored under tiny assessment. Microscopic organisms cell walls are stained by the precious stone violet. Iodine is along these lines added as a severe to shape the precious stone violet-iodine complex so the color can't be expelled effectively.Hence, the correct answer is option b " there is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly