Protein sequencing 2. Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data.

Amino acid composition: (4S,2L, F,G, I,K, M,T, W,Y)

N-terminal analysis: S

Carboxypeptidase digestion: L

Trypsin digestion: (3S,2L, F,I, M,T, W) (G, K,S, Y)

Chymotrypsin digestion: (F, I,S) (G, K,L) (L, S) (M, T) (S, W) (S, Y)

N-terminal analysis of (F, I,S) peptide: S

Cyanogen bromide treatment: (2S, F,G, I,K, L,M*,T, Y) (2S, L,W)

M*, methionine detected as homoserine