If you wanted to prepare a 1:10,000 dilution of your sample and cannot measure out quantities of less than 1 mL, and have dilution blanks that can hold only 10 mL, how many dilution blanks will you need?

a. 2
b. 3
c. 4
d. 5

4.

Explanation:

The dilution process may be defined as the increasing of the solute concentration by mixing it with water or any other soluble reagents. The dilution is important to detect the bacterial growth.

The four blank is required to make the dilution of 1:10,000. The first blank contains 10⁰ or 1 ml and takes out 10 ml and 90 ml will remain in dilution. This results in 1: 10 ml dilution. Then further subsequent dilution from the second blank results 1:100 ml and tbird dilution contains blanks with 1:100 dilution. The fourth blanks dilution results in 1: 10,000.

Thus, the correct answer is option (c).

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