A DNA sequence produces a mutant protein in which several amino acids in the middle of the protein differ from the normal protein. What kind of mutation could have occurred?

The image shows the evolution of a species of fish. a few fish from a population developed different social behaviors and evolved into different species. two fish according to the image, the fish underwent . the new species of fish had mating seasons that were different from that of the original fish. because of the differences in mating seasons, the fish underwent reproductive isolation. this mode of isolation would be .