EML is a protein that is produced at low levels in healthy cells, but is highly expressed in cancer cells. You know that EML expression correlates with HER2 activity in cancer

cells, but you do not know if EML causes HER2 activation or if HER2 activation induces EML expression.

a. What method would you use to quantify expression of EML (Hint: should involve SYBR green)?

b. After using CRISPR/Cas9 to knock out the HER2 gene in a healthy cell line, you transform this CRISPR-edited HER2 -/- cell line into cancer cells. When comparing the expression of EML in the HER2 -/- vs the wild type cancer (assume the HER2 gene is intact) cell lines, you find that the Ct value of EML is significantly higher in the HER2 -/- cells. What conclusion about the relationship between HER2 and EML does this experiment suggest?