Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of eukaryotic genomes, with 145 bp encircling each nucleosome and approximately 55 bp in linker DNA. When C. elegans chromatin is carefully isolated, stripped of non-histone proteins, and placed in an appropriate buffer, the chromatin decondenses. Suppose researchers mix a sample of this chromatin with a large amount of DNase I that randomly cleaves DNA in regions that are not protected by bound proteins. Next, they remove the nucleosomes, separate the DNA fragments by gel electrophoresis, and stain the fragments with ethidium bromide.
a. Approximately what range of DNA fragment sizes do you expect to see in the stained electrophoresis gel? How many bands will be visible on the gel?
b. Explain the origin of DNA fragments seen in the gel.
Answers: 2
Biology, 22.06.2019 00:30
At which location in earth’s interior does the top density continue to increase as thickness decreases?
Answers: 1
Biology, 22.06.2019 01:00
The intervention of extraterrestrials has been used to explain the bermuda triangle, a region of the atlantic ocean where ships and planes are frequently lost, leaving no evidence behind. how would this explanation best be characterized?
Answers: 1
Biology, 22.06.2019 06:30
Achild is suffering from fever but the doctor cannot immediately pinpoint the alignment on the basis of this one symptom explain why also mention other two such general symptoms
Answers: 2
Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner...
Mathematics, 03.12.2020 20:20
Spanish, 03.12.2020 20:20
Mathematics, 03.12.2020 20:20
English, 03.12.2020 20:20
Mathematics, 03.12.2020 20:20
Arts, 03.12.2020 20:20
Mathematics, 03.12.2020 20:20
English, 03.12.2020 20:20