Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads. This method of affinity chromatography would be expected to collect which of the following? A. Molecules of the hormone. B. Molecules of purified receptor. C. G protein. D. Assorted membrane proteins. E. Hormone-receptor complexes.

Affinity chromatography is a method used to separate molecules with high affinity. This technique enables the purification of specific molecules ranging form nucleic acids, enzymes, antigens, antibodies, etc. In the example, the ligand will produce the receptor to be displaced from the beads and then eluted out.

lysosomes contain a variety of enzymes, enabling the cell to break down various biomolecules it engulfs, including peptides, nucleic acids, carbohydrates, and lipids (lysosomal lipase). the enzymes responsible for this hydrolysis require an acidic environment for optimal activity. hope i !

the correct answer is c   with the systems