You have a collaborator who thinks she has created a compound to bind and inhibit an enzyme involved in lung cancer metastasis.
1. Which of the following experiments using lysate from a tumor should be considered to test whether or not this drug would bind to the protein?
Choose one:
A. Perform a size-exclusion chromatography with lysate both with and without the compound and analyze the elution fraction for changes in size.
B. Perform an immunoprecipitation using antibodies against the protein of interest after adding the drug compound to the lysate.
C. Mix the compound with the lysate and then perform and NMR spectroscopy of the mixture.
D. Fix the drug compound to a chromatography bead and perform an affinity chromatography on the lysate to detect the protein that binds the drug.