You are interested in examining the different kinetics of two isozymes of malate dehydrogenase (MDH). Isozymes are two similar enzymes that catalyze the same reaction but are from different genes or splice variants, giving them different properties. One of the MDH isozymes is located in the mitochondria, whereas the other is located in the cytoplasmic fraction. Your goal is to simply separate the two isozymes and test the samples in the partially purified state.