Martin is studying sucrase activity. he added sucrose solution + sucrase solution + buffer of ph 5.6 in two tubes. he incubated them at 350c and 1000c respectively. when he performed a confirmatory test, only the first test tube showed enzyme activity. how would you justify the inactivity of the enzyme in the second test tube?

the enzymes in the second test tube must have denatured at ph 5.6, since most enzymes work at neutral ph.

the enzymes in the second test tube must have denatured at 1000c, since most enzymes degrade at such high temperatures.

enzymes must have remained dormant at 1000c, since most of the enzymes remain inactive at such higher temperatures.