One method for separating polypeptides utilizes their different solubilities. The solubility of large polypeptides in water depends upon the relative polarity of their R groups, particularly the number of ionized groups. The more ionized groups there are, the more soluble the polypeptide. Which polypeptide, ( Phe − Met ) 3 or ( Glu ) 20 , is more soluble at pH 7.0 ?
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One method for separating polypeptides utilizes their different solubilities. The solubility of larg...
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