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Biology, 10.03.2020 04:11 chefjones06p0gvlh

As noted in the text, the OmpF porin in E. coli has a constricted diameter that prevents the passage of large substances. The protein loops in this constricted site contain a D-E-K-A sequence, which makes the porin weakly selective for cations. If you wanted to construct a mutant porin that was highly selective for calcium ions, what changes would you make to the amino acid sequence at the constricted site?

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