How do you isolate and copy the COI gene from your salmon sample?
Once you have obtained your...
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Biology, 10.03.2020 16:26 AikawaAisyah2080
How do you isolate and copy the COI gene from your salmon sample?
Once you have obtained your salmon sample from a local store, you need to isolate the DNA from it and make many copies of the COI gene using the polymerase chain reaction (PCR).
Place the following steps of the DNA extraction and PCR process in the correct order. Not all labels will be used.
1. Disrupt cell, nuclear, and organelle membranes to release their contents into solution.
2. Add Tag, primers, and dNTPs to muscle tissue.
3. Cut out a clean section of muscle tissue.
4. Add Tag, primers, and dNTPs to the DNA sample.
5. Use a thermal cycler to denature, anneal, and extend the target sequence (the COI gene) in 35 cycles.
6. Eliminate non-DNA molecules using wash buffers.
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