In an enzyme-mediated reaction, the activation energy will be the activation energy required for a reaction without an enzyme.

I think that the answer energy reacted and the activation energy required for a reaction with an enzyme

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.