An actively respiring bacterial culture is briefly incubated with [1-14C] glucose (glucose labeled with C radioisotope at carbon 1), and the glycolytic and citric acid cycle intermediates are isolated. a. On a separate piece of paper to be turned in with your homework, draw glycolysis and one turn of the citric acid cycle using [1-14C] glucose and clearly show the 14C label in each step. b. State where the lºc label is in each of the intermediates listed below. Consider only the initial incorporation of "C, in the first pass of labeled glucose through the pathway.

i. Fructose-1,6-bisphosphate
ii. Glyceraldehyde-3-phosphate
iii. Phosphoenolpyruvate
iv. Acetyl-CoA
v. Citrate
vi. a-ketoglutarate
vii. Oxaloacetate

i) C-1ii) C-3iii) C-3iv) C-2 (methyl group)v) C-4vi) C-4vii) Equally distributed in C-2 and C-3.

Explanation:

i) In  Fructose-1,6-bisphosphate , initial incorporation of 14^C occurs at C-1

ii) In Glyceraldehyde-3-phosphate, initial incorporation of 14^C occurs at C-3

iii) In Phosphoenolpyruvate, initial incorporation of 14^C occurs at C-3

iv) In Acetyl-CoA, initial incorporation of 14^C occurs at C-2 (methyl group)

v) In Citrate, initial incorporation of 14^C occurs at  C-4

vi) In a-ketoglutarate, initial incorporation of 14^C occurs at C-4

vii) in Oxaloacetate, initial incorporation of 14^C occurs at Equally distributed in C-2 and C-3.

We can plant more trees and plants.

Environmental science studies the interactions between the physical, chemical, and biological components of the environment, including their effects on all types of organisms. earth science (also known as geoscience), is an inclusive term for all sciences related to earth (geology, meteorology, oceanogr maphy, etc).  although environmental and earth science cover essentially the same material, environmental science places greater emphasis on the biological realm, while earth science places greater emphasis on the physical realm.    environmental and earth science study the interactions of four major systems or “spheres” (figure 8.6).  the  geosphere  consists of the core, mantle and crust of the earth.the  atmosphere  contains all of the earth’s air and is divided into troposphere, stratosphere, mesosphere, thermosphere and ionosphere.the  hydrosphere  contains all of the solid, liquid and gaseous water on earth, extending from the depths of the sea to the upper reaches of the troposphere where water is found.  ninety-seven percent of the hydrosphere is found in salty oceans, and the remainder is found as vapor or droplets in the atmosphere and as liquid in ground water, lakes, rivers, glaciers and snowfields.  the  biosphere  is the collection of all earth’s life forms, distributed in major life zones known as biomes:   tundra, boreal forest, temperate deciduous forest, temperate grassland, desert, savannah, tropical rainforest, chaparral, freshwater, and marine.although the four systems have their unique identities, there is substantial interaction between them.  environmental scientists study the effects of events in one sphere on the other spheres. for example, a volcanic eruption in the geosphere may cause profound direct and indirect effects on the hydrosphere, atmosphere and biosphere as follows: example 1 (volcano)    on may 18, 1980, mount saint helens, in the state of washington, erupted.  this event altered the surrounding environment, and provided scientists with an opportunity to study the effects of volcanic eruptions on the geosphere, hydrosphere, atmosphere and biosphere.  such studies are vital because volcanic eruptions will continue to occur, and will have increasing impact on humans as people continue to settle lands closer to dormant volcanoes. the following are but a few of the myriad of interactions resulting from a volcanic