Shown below is the start of a coding region within the first exon of a gene. 5'--3' 3'--5' What is the PAM sequence of the bottom strand of DNA?

3'-GGT-5'

Explanation:

Protospacer adjacent motif or PAM is an important part of the CRISPR/Cas 9 gene editing system.CRISPR/Cas 9 is a popular gene editing tool that uses an RNA guided endonuclease that cuts DNA at precise target points. As shown in the picture, the CRISPR/Cas9 system consists of the following parts:Cas9 endonucleaseGuide RNA complementary to the target sequence.PAM sequence - a 3 nucleotide sequence on the guide RNA that helps the Cas9 in recognizing and binding to the target DNA. PAM sequences are located 3-6 nucleotides downstream of the target DNA.Every endonuclease recognizes a different PAM sequence. However, the most commonly used Cas9 from Streptococcus pyogenes recognizes the sequence 5' NGG 3'. The "N" represents any nucleotide.

Where’s the selection?