Several lab groups independently carried out similar ligation/transformation steps that you
pe...
Several lab groups independently carried out similar ligation/transformation steps that you
performed in the lab to introduce GFP into the MCS of the phosphatase treated digested pUC
vector. Both the vector and GFP sequence had been digested with the same single enzyme. The
results obtained by each of the groups were as follows:
# of colonies on LB-Amp plates
Transformation into XL-1 Group 1 Group 2 Group 3 Group 4
a) Cut pUC + GFP insert 0 529 2 1125
b) Cut pUC 0 2 5 930
c) GFP alone 0 0 0 890
d) 10 uL uncut pUC (0.5 ng/uL) 0 975 975 975
e) No DNA 0 0 0 925
6. Which group obtained the expected/desired pattern of results?
7. Which group would be expected to give the greatest percentage of blue colonies on X-Gal
plates with the transformation mixture "a"?
8. Which transformation mixture would be expected to have the highest percentage of
intramolecular ligation events?
9. What was the transformation efficiency of the competent XL-1 cells? Indicate the number of
transformants expected per microgram of undigested DNA.
Answers: 3
Biology, 21.06.2019 16:40
Which of the following is a cause of a dna mutation? o a. the linking of amino acid subunits into a long chain o b. the transcription of dna into rna o c. an error in the repair of damaged dna d. the diffusion of molecules across the cell membrane submit
Answers: 1
Biology, 22.06.2019 03:50
What did not occur in the proterozoic eon a)the oxygen levels increase greatly in the atmosphere,b)the emergence of the eukarya , c)muti cella’s eukaryotic organisms began to diversify,d)anaerobic bacteria kept thriving with more oxygen
Answers: 1
Biology, 22.06.2019 04:00
Plsss > .< compare and contrast the characteristics of flatworms rounds worms and earthworms
Answers: 1
Biology, 22.06.2019 05:00
Match each term to its best definition? sedimentary depositional enviornment
Answers: 1
Social Studies, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
English, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Social Studies, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40
Mathematics, 13.04.2021 21:40